Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling
Regulatory protein phosphorylation controls normal and pathophysiological signaling in eukaryotic cells.Despite great advances in mass-spectrometry-based proteomics, the extent, localization, and site-specific stoichiometry of this posttranslational modification (PTM) are unknown.Here, we develop a stringent experimental and computational workflow,